UMAP

Cluster (0.1)

Sample

No integration needed.

Markers

resolution : 0.1
total 5 clusters

Table

Marker genes dotplot

Cluster annotation

cluster0 : Antigen presenting cell + macrophage
cluster1 : Neutrophil
cluster2 : T cell
cluster3 : Macrophage
cluster4 : Antigen presenting cell

Cluster2 : clonotype-associated cluster

Marker genes Dotplot Extended (vertical)

Genes were selected by Sung.

Marker genes Dotplot Extended (horizontal)

Heatmap

DEGs between ADU/CTL

Number of Cells

Significant genes cutoff

FDR=0.05
fold change=1.2

Cluster0

Cluster1

Cluster2

Cluster3

Cluster4



GSEA of DEGs

Hallmark pathway enrichment

NES Plot Cluster3 : Macrophage

NES Plot Cluster0

NES Plot Cluster1

NES Plot Cluster2

NES Plot Cluster4

Comparison of Interferon genes

Interferon-related genes

Isg15, Isg20, Ifitm3, Ifit1, Ifit3, Ifngr1, Irf7, Irf5, Irf8, Irf3

Cluster 3

Cluster 0

Cluster 1

Cluster 2

Volcanoplot

Cluster 3

Cluster 0

Cluster 1

Cluster 2

Cluster 4